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ELECTRON MICROSCOPY RESEARCH |
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The
Glaucoma Research Cellular Ultrastructure and Function Laboratories
use a scanning electron microscope to discover the underlying mechanisms
of ocular disease. Scanning electron microscopy examines structure by bombarding the specimen with a scanning beam of electrons and then collecting slow moving secondary electrons that the specimen generates. These are collected, amplified, and displayed on a cathode ray tube. The electron beam and the cathode ray tube scan synchronously so that an image of the surface of the specimen is formed.*
The Glaucoma Research Cellular Ultrastructure and Function Laboratories use a transmission electron microscope to view mitochrondria and other cellular structural elements.
The (TEM) microscope is used to examine the intracellular conditions in various types of glaucoma, both invivo and invitro. Transmission Electron microscopes are used to penetrate the surface structures of intracellular elements.
*Descriptions
of SEM & TEM functions are courtesy of |
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©2008
University of California, San Francisco, Department of Ophthalmology
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Transmission
electron microscopy examines structure by passing electrons through the
specimen. The image is formed as a shadow of the specimen on a phosphorescent
screen. In order for electrons to pass through the specimen, it must be
very thin (usually less than 100 nanometers or approx. 1/25,000 inch)
thick.*