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Jennifer LaVail,
Ph.D
phone: (415) 476-1694
email: jhl@itsa.ucsf.edu
Understanding
the mechanisms of intraaxonal transport of neurotropic viruses is
the key to understanding and controlling their spread between the
epithelial surface and the nervous system. A second focus of the
lab is to understand the mechanisms by which Herpes virus spreads
within and between corneal cells.
Specific research areas include:
1)
Mechanism that control the assembly and transport of Herpes simplex
virus in neurons.
2) Real-time imaging of the movement of GFP-labeled viral protein
in axons.
3) Host cell and viral glycoproteins that direct the spread of virus
in corneal epithelium.
4) Factors that affect viral patterns of encephalitis.
To
carry out these studies, we have identified two viral mutant strains
that will facilitate our research into these transport mechanisms.
We analyze their behaviors using cell culture, immunohistochemistry,
electron microscopy and protein chemistry. We are looking for
UCSF and visiting medical and graduate students to join our group
for long-term projects as well as for 3 month projects.
Selected
publications
Ohara, P.T., Chin, M.S. and J.H.
LaVail. The spread of Herpes Simplex Virus type 1 from trigeminal
neurons to cornea: an immunoelectron microscopy study. J.Virol.
74:4776-4786, 2000.
Bearer, E.L., X.O. Breakefield, D. Schuback, T.S. Reese and J.H.
LaVail. Retrograde axonal transport of herpes simplex virus: evidence
for a single mechanism and a role for tegument. P.N.A.S. (USA),
97 (14): 8146-8150, 2000
Ohara, P.T., Tauscher, A.N. and J.H. LaVail. Two paths for dissemination
of Herpes simplex virus from infected trigeminal ganglion to murine
cornea. Brain Res. 899: 260-263, 2001.
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Todd P.
Margolis M.D., Ph.D.
email: tpms@itsa.ucsf.edu
Our laboratory is primarily interested
in neuronal regulation of latent infection with Herpes Simplex
Virus.
Specific research areas include:
1) Identifying and characterizing neuronal gene products that
repress productive HSV infection.
2) Differences in the regulation of latent infection of HSV-1
and
HSV-2.
3) The development of a novel model for studying spontaneous
reactivation of HSV-1 from latent infection.
Research studies are conducted using techniques ranging from basic
molecular methods, to cell culture systems to mouse models of HSV
ocular disease. We also make extensive use of fluorescence and
confocal microscopy and in situ hybridization. Research opportunities
are available to UCSF and visiting students usually as 12 month
projects, although 3 month or summer research projects will also
be considered.
We have recently expanded our research interests to include the
study of the molecular genetics of corneal dystrophies.
Selected
publications
Yang L, CC Voytek and TP Margolis. Immunohistochemical Analysis
of Primary
Sensory Neurons Latently Infected with Herpes Simplex Virus. J. Virol 74:209-217,
2000.
Ellison A, L Yang and TP Margolis: Establishment of Latent
Herpes SimplexVirus type 1 Infection in Resistant, Sensitive and
Immunodeficient Mouse Strains. Virology 268:17-28, 2000.
Summers BC, TP Margolis and DA Leib: HSV-1 Corneal Infection
Results in Periocular Disease by Zosteriform Spread. J. Virol.
75:5069-5075, 2001.
Feldman LT, AR Ellison, CC Voytek, L Yang, P Krause and TP
Margolis. Molecular Reactivation of HSV-1 Latency in Mouse Trigeminal
Ganglia. Proc. Natl. Acad. Sci. 99:978-83, 2002.
Ellison AR, L Yang, AV Cevallos and
TP Margolis: Analysis of the HSV UL6 Coding Region in Patients with HSV Necrotizing
Stromal
Keratitis. Virology 310: 24-26, 2003.
Warren JF, Aldave AJ, Srinivasan M, Thonar EJ, Kumar AB, Cevallos
V, Whitcher JP,
Margolis TP. Novel mutations in the CHST6 gene associated with macular corneal
dystrophy in southern India. Arch Ophthalmol. 2003 Nov; 121(11): 1608-12.
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